全文获取类型
收费全文 | 312篇 |
免费 | 14篇 |
国内免费 | 32篇 |
专业分类
测绘学 | 2篇 |
大气科学 | 1篇 |
地球物理 | 137篇 |
地质学 | 24篇 |
海洋学 | 162篇 |
综合类 | 23篇 |
自然地理 | 9篇 |
出版年
2024年 | 1篇 |
2023年 | 4篇 |
2022年 | 3篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 1篇 |
2017年 | 17篇 |
2016年 | 16篇 |
2015年 | 7篇 |
2014年 | 21篇 |
2013年 | 13篇 |
2012年 | 12篇 |
2011年 | 19篇 |
2010年 | 17篇 |
2009年 | 23篇 |
2008年 | 26篇 |
2007年 | 20篇 |
2006年 | 13篇 |
2005年 | 11篇 |
2004年 | 19篇 |
2003年 | 9篇 |
2002年 | 18篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1998年 | 9篇 |
1997年 | 10篇 |
1996年 | 9篇 |
1995年 | 7篇 |
1994年 | 9篇 |
1993年 | 4篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 7篇 |
1986年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有358条查询结果,搜索用时 15 毫秒
301.
本试验以大豆饼作为主要蛋白源取代部分优质鱼粉饲养罗非鱼。结果表明,以大豆饼取代50%鱼粉并添加4.8%油脂的饲料是可行的,具有一定的实际意义。而过多地以植物蛋白代替鱼蛋白显然还存在不少问题。 相似文献
302.
303.
牙鲆(Paralichthys olivaceus)红细胞单克隆抗体与五种养殖鱼类血细胞的交叉反应 总被引:1,自引:0,他引:1
以牙鲆血细胞为抗原免疫Balb/c小鼠,选用聚乙二醇(PEG)作为细胞融合剂,将免疫的小鼠脾细胞与P3 X63 Ag8U1骨髓瘤细胞融合,筛选、克隆出3株生产抗牙鲆红细胞单克隆抗体(Mabs)的杂交瘤细胞(1C7、286、3A1)。应用免疫荧光抗体法(IFAT)研究3株单抗与5种常见养殖鱼类(大菱鲆、花鲈、真鲷、许氏平鲉、鲫鱼)红细胞的交叉反应,结果显示,3株单抗与5种鱼类红细胞有不同程度的阳性反应;应用Western blotting法分析单抗识别的蛋白分子量,结果显示,单抗1C7与大菱鲆血细胞结合的蛋白分子量为52kD、47kD、45kD、32kD、29kD、27kD,与花鲈、许氏平 结合的蛋白分子量是52kD、29kD,与真鲷结合的为29kD,与鲫鱼结合的为44kD、29kD、28kD;单抗286与大菱鲆、花鲈血细胞结合的蛋白带分子量是30kD、28kD,与真鲷结合的为30kD,与许氏平 结合的为29kD、28kD;单抗3A1没有Western blotting反应。结果表明,这6种鱼红细胞存在相同或相似的抗原决定簇,蛋白成分具有相似性。 相似文献
304.
We have shown previously that exposure of Atlantic croaker to a PCB mixture (Aroclor 1254) results in impaired reproductive neuroendocrine function. In addition, we have identified hypothalamic tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin (5-hydroxytryptamine) synthesis, as a target of PCB neuroendocrine toxicity. In order to further elucidate the mechanisms of PCB neurotoxicity, the present study investigated whether PCB-induced decrease in hypothalamic TPH activity resulted from degradation of the enzyme protein. Fish were exposed to Aroclor 1254 in the diet (0.1 mg/100 g body weight (BW)/day) for 30 days. The PCB exposure elicited a significant decrease in hypothalamic TPH protein content, which could be at least partially responsible for the reduced TPH activity. To test whether a similar PCB exposure could cause oxidative damage in croaker hypothalamic tissues, we examined the formation of malondialdehyde (MDA) protein adducts as a marker of lipid peroxidation (LPO). The same dose of PCB increased the MDA-protein adduct formation in the hypothalamus. In a separate experiment, the role of vitamin E, an antioxidant, to prevent or decrease the effect of PCB on hypothalamic TPH activity and gonadal growth was examined. The vitamin E co-treatments (1 and 10 mg/100 g BW/day) with PCB significantly reduced the effects of PCB on TPH activity and gonadal growth. These results suggest possible involvement of oxidative processes in PCB neurotoxicity. 相似文献
305.
Abstract. A shallow water fish community in the inner Oslofjord, Norway, was sampled with a beach seine at 4h intervals over two 36 h periods (25-26th May and 28-29th September, 1982). Night length varied from 1 h in May to 11 h in September. Differences in the community composition were observed between May and September. The diversity of the fish community changed between the two months although the interpretation of the change was dependent on the method used to analyse the community. The three dominant species ( Gasterosteus aculeatus, Sprattus sprattus , and Ammodytes tobianus ) showed diel variations in catch rate which were out of phase with each other. The percentage overlap in abundance of the dominant species was low. The summation of the individual periodicities resulted in aperiodicity at the community level. There were fluctuations, over a diel cycle, in all the community parameters (number of individuals, number of species, species diversity [H'J, species richness [D] and evenness [J']) which affect measures of resource partitioning in fish communities and which should therefore be considered in ecological studies. Diel fluctuations in abundance of some individual shallow water species varied between seasons causing problems in population estimates. 相似文献
306.
The presence of multiple distinct UGT genes in fish was derived by analysis of DNA sequence data derived the zebrafish EST project, confirming indications from previous protein purification studies in another fish species, the plaice, for a diversity of isoforms in lower vertebrates. At least 10 different UGTs can be identified from nucleotide sequence data in zebrafish. Phylogenetic analysis of exon 1 sequences of the zebrafish, plaice and human UGTs indicates that six of these genes are related to the 1A, 1B and 2 families and that a further four genes were of more ancient lineage. Importantly data for the 3' sequences of the zebrafish clones, both from the database and our own sequences of the publicly available clones did not provide any evidence for elaboration of family 1A genes by alternative splicing in this lower vertebrate. 相似文献
307.
308.
309.
厦门港及邻近海域的浮性鱼卵和仔,稚鱼 总被引:6,自引:4,他引:6
本文报道厦门岛区和大嶝岛区海域浮性鱼卵和仔,稚鱼的种类组成与数量分布。其记录44种鱼类,其中有8个主要种类,鱼卵和仔,稚鱼和总密度季节变化较大,均以冬,春季高,夏秋季低。 相似文献
310.
温度和活动对仔鲱氧代谢的影响 总被引:7,自引:0,他引:7
于1993年春在苏格兰采用微电量测氧仪测定大西洋鲱仔鱼在封闭式呼吸器内的耗氧量,并以其作代谢率,通过红外光源录象系统同步纪录仔鲱的游泳活动,从而首次实现仔全在不同温度条件下代谢与活动的相关。同时,还分别测定仔鲱在浅麻醉(静止)和黑暗状态下的耗氧量,仔鲱的实验和饲养温度一致,分为7.3,11.0和12.9℃3个组别。结果表明,仔鲱在常规,静止和黑暗条件下的耗氧量均与体重显著相关,且随温度而提高。仔鲱 相似文献